Hotspot

rpl32-trnL-UAG

SSC · 112373-113126 · 754 bp

Back to Nymphoides

Pi 0.0275

MarkerSeek score 73.10

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 754
variable_sites: 47
pi: 0.0275
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TGTGCGACAAACAAATAAATCA	AGCGTGTCTACCGATTTCAC	31.8	50.0	55.5	58.0	808-838 bp	1.0	52.4
rpl32-trnL-UAG_p2	TGTGCGACAAACAAATAAATCA	CAGCGTGTCTACCGATTTCA	31.8	50.0	55.5	58.0	809-839 bp	1.0	52.4
rpl32-trnL-UAG_p3	TGTGCGACAAACAAATAAATCA	TCGAACCGAGATGCTTTAGC	31.8	50.0	55.5	58.1	844-874 bp	1.0	51.5
rpl32-trnL-UAG_p4	TTTTTGTGCGACAAACAAAT	AGCGTGTCTACCGATTTCAC	30.0	50.0	53.4	58.0	812-842 bp	1.0	51.5
rpl32-trnL-UAG_p5	TTTTTGTGCGACAAACAAAT	CAGCGTGTCTACCGATTTCA	30.0	50.0	53.4	58.0	813-843 bp	1.0	51.5