Hotspot

petA-psbJ

LSC · 62768-63779 · 1012 bp

Back to Nymphoides

Pi 0.0311

MarkerSeek score 71.70

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1012
variable_sites: 67
pi: 0.0311
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	AGAAACAGTTCGAGAAGGTTCA	ATTCCGGATTGGGTTCATCC	40.9	50.0	57.6	57.6	1080-1105 bp	1.0	63.5
petA-psbJ_p2	TCGAGAAGGTTCAATTGTCTGA	ATTCCGGATTGGGTTCATCC	40.9	50.0	57.4	57.6	1071-1096 bp	1.0	62.0
petA-psbJ_p3	GAAACAGTTCGAGAAGGTTCA	ATTCCGGATTGGGTTCATCC	42.9	50.0	56.1	57.6	1079-1104 bp	1.0	59.9
petA-psbJ_p4	CAGTTCGAGAAGGTTCAATTGTC	ATTCCGGATTGGGTTCATCC	43.5	50.0	58.0	57.6	1075-1100 bp	1.0	58.6
petA-psbJ_p5	GTTCGAGAAGGTTCAATTGTCTG	ATTCCGGATTGGGTTCATCC	43.5	50.0	58.0	57.6	1073-1098 bp	1.0	58.6