Hotspot

accD-psaI

LSC · 58468-59165 · 698 bp

Back to Nymphoides

Pi 0.0186

MarkerSeek score 65.30

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 698
variable_sites: 30
pi: 0.0186
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GAGTTATTTCAGCTCCACGC	GGCACCAAAACAGAGGGAAA	50.0	50.0	56.9	58.6	689-817 bp	1.0	73.6
accD-psaI_p2	GAGTTATTTCAGCTCCACGC	GCACCAAAACAGAGGGAAAG	50.0	50.0	56.9	57.2	688-816 bp	1.0	71.9
accD-psaI_p3	GAGTTATTTCAGCTCCACGC	GGCACCAAAACAGAGGGAA	50.0	52.6	56.9	57.9	689-817 bp	1.0	69.3
accD-psaI_p4	TGAGTTATTTCAGCTCCACGC	GGCACCAAAACAGAGGGAAA	47.6	50.0	58.7	58.6	690-818 bp	1.0	69.1
accD-psaI_p5	TCCAGAAGGGCTTATTCGATC	GGCACCAAAACAGAGGGAAA	47.6	50.0	57.2	58.6	748-876 bp	1.0	68.1