Hotspot

ndhC-trnV-UAC

LSC · 50539-51293 · 755 bp

Back to Nymphoides

Pi 0.0168
MarkerSeek score 67.10
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
755
variable_sites
32
pi
0.0168
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.99
markerseek_score
67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TAATCGGCGCTAAAACTCCC CGGTTCAAGTCCGTATAGCC 50.0 55.0 58.1 58.2 868-881 bp 1.0 82.5
ndhC-trnV-UAC_p2 TAATCGGCGCTAAAACTCCC GGTTCAAGTCCGTATAGCCC 50.0 55.0 58.1 57.8 867-880 bp 1.0 81.9
ndhC-trnV-UAC_p3 CCTTTCCTAATCGGCGCTAA CGGTTCAAGTCCGTATAGCC 50.0 55.0 57.8 58.2 875-888 bp 1.0 81.1
ndhC-trnV-UAC_p4 CCTTTCCTAATCGGCGCTAA GGTTCAAGTCCGTATAGCCC 50.0 55.0 57.8 57.8 874-887 bp 1.0 80.4
ndhC-trnV-UAC_p5 TCCTTTCCTAATCGGCGCTA CGGTTCAAGTCCGTATAGCC 50.0 55.0 58.6 58.2 876-889 bp 1.0 78.2