Hotspot

trnG-GCC-trnfM-CAU

LSC · 36624-36808 · 185 bp

Back to Nymphoides

Pi 0.0346

MarkerSeek score 72.80

Primer available yes

Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name: trnG-GCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 185
variable_sites: 12
pi: 0.0346
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnfM-CAU_p1	TCTTTGCCAAGGAGAAGACG	CTTGAGGTCACGGGTTCAAA	50.0	50.0	57.8	58.0	255-278 bp	1.0	85.9
trnG-GCC-trnfM-CAU_p2	AATGCGGATATGGTCGAGTG	CTTGAGGTCACGGGTTCAAA	50.0	50.0	57.8	58.0	285-308 bp	1.0	85.7
trnG-GCC-trnfM-CAU_p3	TCTTTGCCAAGGAGAAGACG	GGGTAGAGCAGTTTGGTAGC	50.0	55.0	57.8	58.0	291-314 bp	1.0	85.6
trnG-GCC-trnfM-CAU_p4	AATGCGGATATGGTCGAGTG	GGGTAGAGCAGTTTGGTAGC	50.0	55.0	57.8	58.0	321-344 bp	1.0	85.5
trnG-GCC-trnfM-CAU_p5	AATGCGGATATGGTCGAGTG	GTCACGGGTTCAAATCCTGT	50.0	50.0	57.8	58.1	279-302 bp	1.0	85.2