Hotspot

psbM-trnD-GUC

LSC · 29936-30924 · 989 bp

Back to Nymphoides

Pi 0.0141
MarkerSeek score 65.30
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
989
variable_sites
33
pi
0.0141
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 GGAACTAAAATGAACAGTGCAGT TTCAATTGGTCAGAGCACCG 39.1 50.0 57.6 58.5 1104-1137 bp 1.0 52.5
psbM-trnD-GUC_p2 AAAATGAACAGTGCAGTAGC TTCAATTGGTCAGAGCACCG 40.0 50.0 54.5 58.5 1098-1131 bp 1.0 52.1
psbM-trnD-GUC_p3 ACTAAAATGAACAGTGCAGTAGC TTCAATTGGTCAGAGCACCG 39.1 50.0 57.4 58.5 1101-1134 bp 1.0 51.1
psbM-trnD-GUC_p4 AGGAACTAAAATGAACAGTGCAG TTCAATTGGTCAGAGCACCG 39.1 50.0 57.3 58.5 1105-1138 bp 1.0 50.4
psbM-trnD-GUC_p5 AGGAACTAAAATGAACAGTGCA TTCAATTGGTCAGAGCACCG 36.4 50.0 56.2 58.5 1105-1138 bp 1.0 49.5