Hotspot

trnS-GCU-trnG-UCC

LSC · 8341-9005 · 665 bp

Back to Nymphoides

Pi 0.0232

MarkerSeek score 67.90

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 665
variable_sites: 33
pi: 0.0232
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CTTTCGTCCACTCAGCCATC	TACCACTAAACTATACCCGC	55.0	45.0	58.6	54.0	706-762 bp	1.0	50.5
trnS-GCU-trnG-UCC_p2	CTTTCGTCCACTCAGCCATC	TTACCACTAAACTATACCCGC	55.0	42.9	58.6	54.9	707-763 bp	1.0	49.4
trnS-GCU-trnG-UCC_p3	TCCACTCAGCCATCTCTCC	TACCACTAAACTATACCCGC	57.9	45.0	58.1	54.0	700-756 bp	1.0	47.7
trnS-GCU-trnG-UCC_p4	CTTTCGTCCACTCAGCCATC	TTTACCACTAAACTATACCCGC	55.0	40.9	58.6	55.6	708-764 bp	1.0	47.6
trnS-GCU-trnG-UCC_p5	TTTCGTCCACTCAGCCATCT	TACCACTAAACTATACCCGC	50.0	45.0	59.0	54.0	705-761 bp	1.0	47.6