Hotspot

psbK-psbI

LSC · 7554-7998 · 445 bp

Back to Nymphoides

Pi 0.0149

MarkerSeek score 68.10

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 445
variable_sites: 16
pi: 0.0149
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	ACGTCCCGGATCATTGGATA	50.0	50.0	57.9	58.3	531-565 bp	1.0	83.5
psbK-psbI_p2	GTTTGGCAAGCTGCTGTAAG	TACGTCCCGGATCATTGGAT	50.0	50.0	57.9	58.3	532-566 bp	1.0	83.5
psbK-psbI_p3	GTTTGGCAAGCTGCTGTAAG	CGTCCCGGATCATTGGATAG	50.0	55.0	57.9	57.6	530-564 bp	1.0	82.9
psbK-psbI_p4	GTTTGGCAAGCTGCTGTAAG	TTACGTCCCGGATCATTGGA	50.0	50.0	57.9	58.5	533-567 bp	1.0	81.7
psbK-psbI_p5	TGGCAAGCTGCTGTAAGTTT	ACGTCCCGGATCATTGGATA	45.0	50.0	58.3	58.3	528-562 bp	1.0	81.5