Hotspot

trnH-GUG-psbA

LSC · 98-580 · 483 bp

Back to Nymphoides

Pi 0.0423
MarkerSeek score 78.70
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
483
variable_sites
44
pi
0.0423
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.98
markerseek_score
78.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG TCGAAGCTCCATCTACAAATGG 55.0 45.5 58.1 58.2 508-539 bp 1.0 72.1
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA TCGAAGCTCCATCTACAAATGG 50.0 45.5 57.9 58.2 522-553 bp 1.0 71.9
trnH-GUG-psbA_p3 AATCCACTGCCTTGATCCAC TCGAAGCTCCATCTACAAATGG 50.0 45.5 57.9 58.2 521-552 bp 1.0 71.9
trnH-GUG-psbA_p4 GATCCACTTGGCTACATCCG CGAAGCTCCATCTACAAATGG 55.0 47.6 58.1 56.8 507-538 bp 1.0 71.9
trnH-GUG-psbA_p5 ACAATCCACTGCCTTGATCC TCGAAGCTCCATCTACAAATGG 50.0 45.5 57.9 58.2 523-554 bp 1.0 71.8