Hotspot

ycf1

SSC · 128979-134909 · 5931 bp

Back to Nymphaea

Pi 0.0233

MarkerSeek score 59.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5931
variable_sites: 518
pi: 0.0233
unique_haplotype_count: 26
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTCTCTTTTGCCCCTTCACC	GGCCAGATTGTGCAATGATG	50.0	50.0	58.0	57.8	1008-1332 bp	1.0	90.2
ycf1_p2	TTCTCTTTTGCCCCTTCACC	AAAAACTACCTGCGGAGGAC	50.0	50.0	58.0	57.8	1171-1471 bp	0.258065	57.6
ycf1_p3	CCGCATCGACCCTTTGATTA	AAAAACTACCTGCGGAGGAC	50.0	50.0	58.1	57.8	980-1271 bp	0.258065	57.2
ycf1_p4	TATCCGTTCCTCCTCCTGTC	AAAAACTACCTGCGGAGGAC	55.0	50.0	57.9	57.8	1253-1553 bp	0.258065	57.1
ycf1_p5	CTATCCGTTCCTCCTCCTGT	AAAAACTACCTGCGGAGGAC	55.0	50.0	57.9	57.8	1254-1554 bp	0.258065	57.1