Hotspot

ndhF-rpl32

SSC · 117480-118619 · 1140 bp

Back to Nymphaea

Pi 0.0308

MarkerSeek score 68.10

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1140
variable_sites: 120
pi: 0.0308
unique_haplotype_count: 22
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GCATATTGATATGTGCGTTCCA	TTTACCGCTGCCCAATATCC	40.9	50.0	57.3	58.0	1189-1278 bp	1.0	69.6
ndhF-rpl32_p2	GCATATTGATATGTGCGTTCCA	AAAAAGCTTTTACCGCTGCC	40.9	45.0	57.3	57.9	1197-1286 bp	1.0	68.6
ndhF-rpl32_p3	GCATATTGATATGTGCGTTCCA	GCTGCCCAATATCCCTTTCT	40.9	50.0	57.3	57.6	1183-1272 bp	1.0	66.9
ndhF-rpl32_p4	GCATATTGATATGTGCGTTCCAT	TTTACCGCTGCCCAATATCC	39.1	50.0	57.7	58.0	1189-1278 bp	1.0	65.2
ndhF-rpl32_p5	GCATATTGATATGTGCGTTCCAT	AAAAAGCTTTTACCGCTGCC	39.1	45.0	57.7	57.9	1197-1286 bp	1.0	64.2