Hotspot

ndhC-tRNA-Val (UAC)

LSC · 54194-56126 · 1933 bp

Back to Nymphaea

Pi 0.0205

MarkerSeek score 66.80

Primer available yes

Label ndhC-tRNA-Val (UAC)

Feature Metadata

label_name: ndhC-tRNA-Val (UAC)
feature_type: igs
region: LSC
length_bp: 1933
variable_sites: 158
pi: 0.0205
unique_haplotype_count: 24
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-tRNA-Val (UAC)_p1	TGCCCAGAAAATATCATATTCGT	GTTCGAGTCCGTATAGCCCT	34.8	55.0	56.1	58.7	1960-2071 bp	1.0	50.3
ndhC-tRNA-Val (UAC)_p2	TGCCCAGAAAATATCATATTCGT	AGAAGGTCTACGGTTCGAGT	34.8	50.0	56.1	57.8	1972-2083 bp	1.0	50.2
ndhC-tRNA-Val (UAC)_p3	TGCCAAAATAGGAATAACACTTGA	AGAAGGTCTACGGTTCGAGT	33.3	50.0	56.8	57.8	2008-2119 bp	1.0	50.2
ndhC-tRNA-Val (UAC)_p4	TGCCCAGAAAATATCATATTCGT	GAAGGTCTACGGTTCGAGTC	34.8	55.0	56.1	57.5	1971-2082 bp	1.0	50.2
ndhC-tRNA-Val (UAC)_p5	ATGCCCAGAAAATATCATATTCGT	AGAAGGTCTACGGTTCGAGT	33.3	50.0	56.6	57.8	1973-2084 bp	1.0	50.2