Hotspot

tRNA-Glu (UUC)-tRNA-Thr (GGU)

LSC · 34160-34723 · 564 bp

Back to Nymphaea

Pi 0.0231

MarkerSeek score 68.60

Primer available yes

Label tRNA-Glu (UUC)-tRNA-Thr (GGU)

Feature Metadata

label_name: tRNA-Glu (UUC)-tRNA-Thr (GGU)
feature_type: igs
region: LSC
length_bp: 564
variable_sites: 53
pi: 0.0231
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 68.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Glu (UUC)-tRNA-Thr (GGU)_p1	TTCGTCGTACCCTACTACCC	GAACCGATGACTTACGCCTT	55.0	50.0	58.0	58.0	668-754 bp	1.0	89.6
tRNA-Glu (UUC)-tRNA-Thr (GGU)_p2	TTCGTCGTACCCTACTACCC	TACCGCTGAGTTAAAAGGGC	55.0	50.0	58.0	57.9	633-719 bp	1.0	89.2
tRNA-Glu (UUC)-tRNA-Thr (GGU)_p3	TTCGTCGTACCCTACTACCC	GCGGAAAAATGACCATTGCA	55.0	45.0	58.0	57.9	717-803 bp	1.0	88.9
tRNA-Glu (UUC)-tRNA-Thr (GGU)_p4	TTCGTCGTACCCTACTACCC	TGCGGAAAAATGACCATTGC	55.0	45.0	58.0	57.9	718-804 bp	1.0	88.9
tRNA-Glu (UUC)-tRNA-Thr (GGU)_p5	TTCGTCGTACCCTACTACCC	ACCATTGCAGCTTCATGGAA	55.0	45.0	58.0	58.1	706-792 bp	0.967742	87.1