Hotspot

petN-psbM

LSC · 31215-32551 · 1337 bp

Back to Nymphaea

Pi 0.0202

MarkerSeek score 65.90

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1337
variable_sites: 101
pi: 0.0202
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ATTCATCGAGATAGGGGGCA	ATTGCTACTGCGCTGTTCAT	50.0	45.0	58.0	58.3	1467-1544 bp	1.0	87.1
petN-psbM_p2	TCGAGATAGGGGGCATGATT	ATTGCTACTGCGCTGTTCAT	50.0	45.0	58.0	58.3	1462-1539 bp	1.0	87.1
petN-psbM_p3	TTCATCGAGATAGGGGGCAT	ATTGCTACTGCGCTGTTCAT	50.0	45.0	58.0	58.3	1466-1543 bp	1.0	87.1
petN-psbM_p4	TCGAGATAGGGGGCATGATT	TATTGCTACTGCGCTGTTCA	50.0	45.0	58.0	57.6	1463-1540 bp	1.0	86.2
petN-psbM_p5	TTCATCGAGATAGGGGGCAT	TATTGCTACTGCGCTGTTCA	50.0	45.0	58.0	57.6	1467-1544 bp	1.0	86.2