Hotspot

atpH-atpI

LSC · 15310-16610 · 1301 bp

Back to Nymphaea

Pi 0.0195

MarkerSeek score 63.50

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1301
variable_sites: 108
pi: 0.0195
unique_haplotype_count: 23
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	GCCAATCCAGCAGCAATAAC	GGTGAATCCATGGAAGGTCA	50.0	50.0	57.8	57.2	1348-1403 bp	1.0	82.1
atpH-atpI_p2	TCCAGCAGCAATAACGGAAG	GGTGAATCCATGGAAGGTCA	50.0	50.0	58.3	57.2	1343-1398 bp	1.0	81.8
atpH-atpI_p3	CCGGGTCCAATAGAAGCAAG	GGTGAATCCATGGAAGGTCA	55.0	50.0	58.3	57.2	1375-1430 bp	1.0	81.1
atpH-atpI_p4	AATACCTTCTACGGCTTGGC	GGTGAATCCATGGAAGGTCA	50.0	50.0	57.7	57.2	1418-1473 bp	1.0	80.9
atpH-atpI_p5	CGGGTCCAATAGAAGCAAGT	GGTGAATCCATGGAAGGTCA	50.0	50.0	57.6	57.2	1374-1429 bp	1.0	80.5