Hotspot

tRNA-Ser (GCU)-tRNA-Gly (UCC)

LSC · 9880-10635 · 756 bp

Back to Nymphaea

Pi 0.0241

MarkerSeek score 71.20

Primer available yes

Label tRNA-Ser (GCU)-tRNA-Gly (UCC)

Feature Metadata

label_name: tRNA-Ser (GCU)-tRNA-Gly (UCC)
feature_type: igs
region: LSC
length_bp: 756
variable_sites: 74
pi: 0.0241
unique_haplotype_count: 22
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 71.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Ser (GCU)-tRNA-Gly (UCC)_p1	GGAAACGGAGAGAGAGGGAT	TGTATCAAACCGAAAGAACCCT	55.0	40.9	58.2	57.6	837-939 bp	0.806452	62.0
tRNA-Ser (GCU)-tRNA-Gly (UCC)_p2	AATCTGCCGCTTTAGTCCAC	TGTATCAAACCGAAAGAACCCT	50.0	40.9	58.3	57.6	778-880 bp	0.806452	62.0
tRNA-Ser (GCU)-tRNA-Gly (UCC)_p3	CAATCTGCCGCTTTAGTCCA	TGTATCAAACCGAAAGAACCCT	50.0	40.9	58.3	57.6	779-881 bp	0.806452	62.0
tRNA-Ser (GCU)-tRNA-Gly (UCC)_p4	AGATTAGCAATCTGCCGCTT	TGTATCAAACCGAAAGAACCCT	45.0	40.9	57.7	57.6	786-888 bp	0.806452	61.4
tRNA-Ser (GCU)-tRNA-Gly (UCC)_p5	CGGAGAGAGAGGGATTCGAA	TGTATCAAACCGAAAGAACCCT	55.0	40.9	58.3	57.6	832-934 bp	0.806452	61.2