Hotspot

psbI-tRNA-Ser (GCU)

LSC · 9615-9791 · 177 bp

Back to Nymphaea

Pi 0.0369

MarkerSeek score 64.90

Primer available yes

Label psbI-tRNA-Ser (GCU)

Feature Metadata

label_name: psbI-tRNA-Ser (GCU)
feature_type: igs
region: LSC
length_bp: 177
variable_sites: 26
pi: 0.0369
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-tRNA-Ser (GCU)_p1	TGTCTAATGACCCAGGACGT	GCTATTCGTACCGAGGGTTC	50.0	55.0	58.1	57.9	253-284 bp	1.0	87.4
psbI-tRNA-Ser (GCU)_p2	TGTCTAATGACCCAGGACGT	GGGTTCGAATCCCTCTCTCT	50.0	55.0	58.1	58.2	239-270 bp	1.0	86.7
psbI-tRNA-Ser (GCU)_p3	TGTCTAATGACCCAGGACGT	AGCTATTCGTACCGAGGGTT	50.0	50.0	58.1	58.2	254-285 bp	1.0	86.6
psbI-tRNA-Ser (GCU)_p4	TGTCTAATGACCCAGGACGT	GTGGACTAAAGCGGCAGATT	50.0	50.0	58.1	58.3	290-321 bp	1.0	85.3
psbI-tRNA-Ser (GCU)_p5	TGTCTAATGACCCAGGACGT	TGGACTAAAGCGGCAGATTG	50.0	50.0	58.1	58.3	289-320 bp	1.0	85.3