Hotspot

rps16-tRNA-Gln (UUG)

LSC · 6413-8477 · 2065 bp

Back to Nymphaea

Pi 0.0242

MarkerSeek score 69.80

Primer available yes

Label rps16-tRNA-Gln (UUG)

Feature Metadata

label_name: rps16-tRNA-Gln (UUG)
feature_type: igs
region: LSC
length_bp: 2065
variable_sites: 199
pi: 0.0242
unique_haplotype_count: 24
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 69.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-tRNA-Gln (UUG)_p1	GTCATTCAAGTCGCACGTTG	ACGGGTTTTGGTCCTGTTAC	50.0	50.0	58.1	58.0	1177-2179 bp	1.0	90.9
rps16-tRNA-Gln (UUG)_p2	GTTGCCTTCTACCACATCGT	ACGGGTTTTGGTCCTGTTAC	50.0	50.0	57.9	58.0	1161-2163 bp	1.0	90.7
rps16-tRNA-Gln (UUG)_p3	ACGTTGCCTTCTACCACATC	ACGGGTTTTGGTCCTGTTAC	50.0	50.0	57.9	58.0	1163-2165 bp	1.0	90.6
rps16-tRNA-Gln (UUG)_p4	AATCCACAGCCGATCATGTC	ACGGGTTTTGGTCCTGTTAC	50.0	50.0	58.0	58.0	1194-2196 bp	0.967742	89.4
rps16-tRNA-Gln (UUG)_p5	AATCCACAGCCGATCATGTC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	1171-2173 bp	0.967742	89.1