Hotspot

tRNA-His (GUG)-psbA

LSC · 79-521 · 443 bp

Back to Nymphaea

Pi 0.035

MarkerSeek score 71.60

Primer available yes

Label tRNA-His (GUG)-psbA

Feature Metadata

label_name: tRNA-His (GUG)-psbA
feature_type: igs
region: LSC
length_bp: 443
variable_sites: 58
pi: 0.035
unique_haplotype_count: 23
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 71.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-His (GUG)-psbA_p1	AATCCACTTGGCTACATCCG	GTCGAAGCTCCATCCACAAA	50.0	50.0	57.7	58.2	471-523 bp	1.0	87.8
tRNA-His (GUG)-psbA_p2	AATCCACTTGGCTACATCCG	CGTGCTAACCTTGGTATGGA	50.0	50.0	57.7	57.3	540-592 bp	1.0	83.9
tRNA-His (GUG)-psbA_p3	AATCCACTTGGCTACATCCG	TCGTGCTAACCTTGGTATGG	50.0	50.0	57.7	57.3	541-593 bp	1.0	83.9
tRNA-His (GUG)-psbA_p4	AATCCACTTGGCTACATCCG	TCGAAGCTCCATCCACAAAT	50.0	45.0	57.7	57.2	470-522 bp	1.0	83.0
tRNA-His (GUG)-psbA_p5	AATCCACTTGGCTACATCCG	CGAAGCTCCATCCACAAATG	50.0	50.0	57.7	57.2	469-521 bp	1.0	82.6