Hotspot

ycf1

SSC · 129867-135473 · 5607 bp

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Pi 0.00473
MarkerSeek score 55.70
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5607
variable_sites
50
pi
0.00473
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 AGAACTATTTTGATAACCCATCT CTTTGGCTCTCCCCGAAAAA 30.4 50.0 53.2 58.4 177 bp 1.0 41.8
ycf1_p2 AGAACTATTTTGATAACCCATCTT CTTTGGCTCTCCCCGAAAAA 29.2 50.0 54.0 58.4 177 bp 1.0 41.8
ycf1_p3 AGAACTATTTTGATAACCCATCT TGGAAACAGAACTGCCCTTT 30.4 45.0 53.2 57.5 193 bp 1.0 41.7
ycf1_p4 AGAACTATTTTGATAACCCATCT GTGGAAACAGAACTGCCCTT 30.4 50.0 53.2 58.3 194 bp 1.0 41.6
ycf1_p5 AGAACTATTTTGATAACCCATCTT GTGGAAACAGAACTGCCCTT 29.2 50.0 54.0 58.3 194 bp 1.0 41.6