Hotspot

rps19

LSC · 90052-90330 · 279 bp

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Pi 0.00358
MarkerSeek score 30.60
Primer available yes
Label rps19

Feature Metadata

label_name
rps19
feature_type
gene
region
LSC
length_bp
279
variable_sites
2
pi
0.00358
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
30.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps19_p1 TGAGCACCTATTTCACTTTT TGAAAAGAAAGGGGAGTAATCACT 35.0 37.5 52.7 57.8 355 bp 1.0 40.7
rps19_p2 TGAGCACCTATTTCACTTTT ACAAAATGAAAAGAAAGGGGAGT 35.0 34.8 52.7 56.8 361 bp 1.0 40.7
rps19_p3 TGAGCACCTATTTCACTTTTT TGAAAAGAAAGGGGAGTAATCACT 33.3 37.5 53.6 57.8 355 bp 1.0 40.7
rps19_p4 TGAGCACCTATTTCACTTTTT ACAAAATGAAAAGAAAGGGGAGT 33.3 34.8 53.6 56.8 361 bp 1.0 40.7
rps19_p5 TGAGCACCTATTTCACTTTT TGAAAAGAAAGGGGAGTAATCAC 35.0 39.1 52.7 56.4 355 bp 1.0 40.7