Pi
0.00253
MarkerSeek score
60.50
Primer available
yes
Label
atpF
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| atpF_p1 | TTTCTTCGGCTCGAATGGTT | TCGGGAATCTAAATAAGGAGTGA | 45.0 | 39.1 | 57.8 | 56.5 | 1507-1512 bp | 1.0 | 43.2 |
| atpF_p2 | TTTCTTCGGCTCGAATGGTT | TCCATAAGATAAGAGGAGAGCA | 45.0 | 40.9 | 57.8 | 55.5 | 1430 bp | 1.0 | 43.0 |
| atpF_p3 | TTTCTTCGGCTCGAATGGTT | AAGTGTCTTATCGGGAATCT | 45.0 | 40.0 | 57.8 | 53.4 | 1517-1522 bp | 1.0 | 42.5 |
| atpF_p4 | TCTTCGGCTCGAATGGTTAC | TCGGGAATCTAAATAAGGAGTGA | 50.0 | 39.1 | 57.7 | 56.5 | 1505-1510 bp | 1.0 | 42.3 |
| atpF_p5 | TCTTCGGCTCGAATGGTTAC | TCCATAAGATAAGAGGAGAGCA | 50.0 | 40.9 | 57.7 | 55.5 | 1428 bp | 1.0 | 42.1 |