Hotspot

rpl14-rpl16

LSC · 87331-87469 · 139 bp

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Pi 0.018
MarkerSeek score 65.90
Primer available yes
Label rpl14-rpl16

Feature Metadata

label_name
rpl14-rpl16
feature_type
igs
region
LSC
length_bp
139
variable_sites
5
pi
0.018
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl14-rpl16_p1 ACATCAGTTTTCGAGCTCCG CAGAGCCGCTATGGAACTAG 50.0 55.0 58.3 57.6 267-280 bp 1.0 76.0
rpl14-rpl16_p2 ACATCAGTTTTCGAGCTCCG TGAAATGGGCGGAGTATCAG 50.0 50.0 58.3 57.4 297-310 bp 1.0 74.4
rpl14-rpl16_p3 ACATCAGTTTTCGAGCTCCG ATGGGCGGAGTATCAGAAAC 50.0 50.0 58.3 57.4 293-306 bp 1.0 74.4
rpl14-rpl16_p4 GCTGTTGTCCGCTACATTCA CAGAGCCGCTATGGAACTAG 50.0 55.0 58.6 57.6 248-261 bp 1.0 74.0
rpl14-rpl16_p5 ACATCAGTTTTCGAGCTCCG GCGCAAAATGCCTATACGAA 50.0 45.0 58.3 57.3 243-256 bp 1.0 73.7