Hotspot

petA-psbJ

LSC · 68686-69911 · 1226 bp

Pi 0.0109

MarkerSeek score 57.80

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1226
variable_sites: 26
pi: 0.0109
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CTGGCGCAAATCTTTTTGGT	CCTGTGATCGGTTTAGTGGG	45.0	55.0	57.8	58.0	1356-1364 bp	1.0	80.6
petA-psbJ_p2	CTGGCGCAAATCTTTTTGGT	ATCGGTTTAGTGGGCGTTTT	45.0	45.0	57.8	58.1	1350-1358 bp	1.0	80.0
petA-psbJ_p3	CTGGCGCAAATCTTTTTGGT	GGCTCATATTCCGGATTGGG	45.0	55.0	57.8	58.2	1323-1331 bp	1.0	79.4
petA-psbJ_p4	CTGGCGCAAATCTTTTTGGT	AGTGGGCGTTTTCTTCTACG	45.0	50.0	57.8	58.2	1342-1350 bp	1.0	79.1
petA-psbJ_p5	TCTGGCGCAAATCTTTTTGG	CCTGTGATCGGTTTAGTGGG	45.0	55.0	57.6	58.0	1357-1365 bp	1.0	78.3