Hotspot

accD-psaI

LSC · 63924-64720 · 797 bp

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Pi 0.0106
MarkerSeek score 54.10
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
797
variable_sites
15
pi
0.0106
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
54.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 GGCGTTCCGAGTGAGTTATT CCATTGCAATTGCCGGAAAT 50.0 45.0 58.0 58.0 911-936 bp 1.0 82.7
accD-psaI_p2 TAAAAGGCGTTCCGAGTGAG CCATTGCAATTGCCGGAAAT 50.0 45.0 57.9 58.0 916-941 bp 1.0 82.1
accD-psaI_p3 TTCCATGGTTTCTTTCCCCG CCATTGCAATTGCCGGAAAT 50.0 45.0 58.1 58.0 887-912 bp 1.0 82.1
accD-psaI_p4 GGCGTTCCGAGTGAGTTATT AAGAAGCCATTGCAATTGCC 50.0 45.0 58.0 57.9 917-942 bp 1.0 82.0
accD-psaI_p5 TCCAATCGTACCGCGTAATC CCATTGCAATTGCCGGAAAT 50.0 45.0 57.9 58.0 939-964 bp 1.0 81.6