Hotspot

trnT-GGU-psbD

LSC · 34987-36540 · 1554 bp

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Pi 0.00377
MarkerSeek score 56.80
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1554
variable_sites
11
pi
0.00377
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
56.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 AACGGGCCCTTTTAACTCAG ACAAAAACGAAACGGTCCCT 50.0 45.0 58.1 58.2 1709-1723 bp 1.0 78.5
trnT-GGU-psbD_p2 GCCCTTTTAACTCAGCGGTA ACAAAAACGAAACGGTCCCT 50.0 45.0 57.9 58.2 1704-1718 bp 1.0 78.4
trnT-GGU-psbD_p3 AACGGGCCCTTTTAACTCAG GACCGGACCAACCTACAAAA 50.0 50.0 58.1 57.7 1723-1737 bp 1.0 78.4
trnT-GGU-psbD_p4 GCCCTTTTAACTCAGCGGTA GACCGGACCAACCTACAAAA 50.0 50.0 57.9 57.7 1718-1732 bp 1.0 78.3
trnT-GGU-psbD_p5 AACGGGCCCTTTTAACTCAG TAGACCGGACCAACCTACAA 50.0 50.0 58.1 57.7 1725-1739 bp 1.0 78.0