Hotspot

atpH-atpI

LSC · 15122-16462 · 1341 bp

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Pi 0.00351
MarkerSeek score 63.50
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
1341
variable_sites
9
pi
0.00351
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.98
markerseek_score
63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 GATACCTTCTACGGCTTGGC ATTTTTGCAACCTTAGCCGC 55.0 45.0 58.1 57.9 1492-1518 bp 1.0 80.5
atpH-atpI_p2 CGATACCTTCTACGGCTTGG ATTTTTGCAACCTTAGCCGC 55.0 45.0 57.9 57.9 1493-1519 bp 1.0 80.5
atpH-atpI_p3 GCCAATCCAGCAGCAATAAC ATTTTTGCAACCTTAGCCGC 50.0 45.0 57.8 57.9 1422-1448 bp 1.0 80.0
atpH-atpI_p4 TCCAGCAGCAATAACGGAAG ATTTTTGCAACCTTAGCCGC 50.0 45.0 58.3 57.9 1417-1443 bp 1.0 79.6
atpH-atpI_p5 GATACCTTCTACGGCTTGGC TACCTGTGATGTTCCTCGGA 55.0 50.0 58.1 57.8 1539-1565 bp 1.0 79.3