Hotspot

rpl16

LSC · 80636-82059 · 1424 bp

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Pi 0.000702

MarkerSeek score 59.20

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1424
variable_sites: 1
pi: 0.000702
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	AAAAAGAAACCCGCACCCG	TCGTTTCTTCTTTTTCTTTTGTGT	52.6	29.2	59.3	56.2	1575 bp	1.0	40.1
rpl16_p2	CCCGCACCCGCTATTTTT	TCGTTTCTTCTTTTTCTTTTGTGT	55.6	29.2	58.4	56.2	1566 bp	1.0	40.1
rpl16_p3	AAAAAGAAACCCGCACCCG	CGTTTCTTCTTTTTCTTTTGTGTTCA	52.6	30.8	59.3	58.0	1574 bp	1.0	40.1
rpl16_p4	AAAAGAAACCCGCACCCG	TCGTTTCTTCTTTTTCTTTTGTGT	55.6	29.2	58.6	56.2	1574 bp	1.0	40.1
rpl16_p5	CCCGCACCCGCTATTTTT	CGTTTCTTCTTTTTCTTTTGTGTTCA	55.6	30.8	58.4	58.0	1565 bp	1.0	40.1