Hotspot

ndhA

SSC · 118515-120661 · 2147 bp

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Pi 0.000932

MarkerSeek score 57.90

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2147
variable_sites: 2
pi: 0.000932
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TGTTTGAGACAAGTCGTGAA	CCACCGGGGTTTATTAATTTGC	40.0	45.5	55.2	58.2	2306 bp	1.0	40.1
ndhA_p2	TTGTTTGAGACAAGTCGTGA	CCACCGGGGTTTATTAATTTGC	40.0	45.5	55.2	58.2	2307 bp	1.0	40.1
ndhA_p3	TTGTTTGAGACAAGTCGTGAA	CCACCGGGGTTTATTAATTTGC	38.1	45.5	56.0	58.2	2307 bp	1.0	40.1
ndhA_p4	TGTTTGAGACAAGTCGTGAA	CACCGGGGTTTATTAATTTGCA	40.0	40.9	55.2	57.5	2305 bp	1.0	40.1
ndhA_p5	TTGTTTGAGACAAGTCGTGA	CACCGGGGTTTATTAATTTGCA	40.0	40.9	55.2	57.5	2306 bp	1.0	40.1