Hotspot

accD

LSC · 56795-58312 · 1518 bp

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Pi 0.00132
MarkerSeek score 59.50
Primer available yes
Label accD

Feature Metadata

label_name
accD
feature_type
gene
region
LSC
length_bp
1518
variable_sites
2
pi
0.00132
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD_p1 GTTCGTAGGAGAGAACAAATCCT ACAAAGAAAACCCCATTCGTC 43.5 42.9 57.9 57.0 1720 bp 1.0 40.1
accD_p2 GTTCGTAGGAGAGAACAAATCCT CAACAAAGAAAACCCCATTCGT 43.5 40.9 57.9 57.9 1722 bp 1.0 40.1
accD_p3 TCGTAGGAGAGAACAAATCCT ACAAAGAAAACCCCATTCGTC 42.9 42.9 55.8 57.0 1718 bp 1.0 40.1
accD_p4 TCGTAGGAGAGAACAAATCCT CAACAAAGAAAACCCCATTCGT 42.9 40.9 55.8 57.9 1720 bp 1.0 40.1
accD_p5 GTTCGTAGGAGAGAACAAATCCT ACAAAGAAAACCCCATTCGT 43.5 40.0 57.9 55.8 1720 bp 1.0 40.1