Hotspot

ndhD-psaC

SSC · 115968-116064 · 97 bp

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Pi 0.0103

MarkerSeek score 58.40

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 97
variable_sites: 1
pi: 0.0103
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	AGGAACCCGCAAATATTGGT	ACAGAGGATTGTGTCGGTTG	45.0	50.0	57.5	57.8	287 bp	1.0	74.7
ndhD-psaC_p2	AGGAACCCGCAAATATTGGT	CAGAGGATTGTGTCGGTTGT	45.0	50.0	57.5	57.8	286 bp	1.0	74.7
ndhD-psaC_p3	AGGAACCCGCAAATATTGGT	AAGAGATGTGAATCCGCCTG	45.0	50.0	57.5	57.7	266 bp	1.0	73.5
ndhD-psaC_p4	AGGAACCCGCAAATATTGGT	CGCAGCATGGGTATAGCTTA	45.0	50.0	57.5	57.5	197 bp	1.0	72.6
ndhD-psaC_p5	AGGAACCCGCAAATATTGGT	GAACAGAGGATTGTGTCGGT	45.0	50.0	57.5	57.5	289 bp	1.0	72.4