Hotspot

rpl32-trnL

SSC · 112093-113013 · 921 bp

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Pi 0.00217

MarkerSeek score 60.00

Primer available yes

Label rpl32-trnL

Feature Metadata

label_name: rpl32-trnL
feature_type: igs
region: SSC
length_bp: 921
variable_sites: 2
pi: 0.00217
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL_p1	AAGGAAAGGATATTGGGCGG	AGCGTGTCTACCGATTTCAC	50.0	50.0	57.6	58.0	1053 bp	1.0	77.2
rpl32-trnL_p2	AAGGAAAGGATATTGGGCGG	CAGCGTGTCTACCGATTTCA	50.0	50.0	57.6	58.0	1054 bp	1.0	77.2
rpl32-trnL_p3	CGGCGTTAAAAGCTTTGTCA	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.6	58.0	1036 bp	1.0	76.9
rpl32-trnL_p4	CGGCGTTAAAAGCTTTGTCA	CAGCGTGTCTACCGATTTCA	45.0	50.0	57.6	58.0	1037 bp	1.0	76.9
rpl32-trnL_p5	AAGGAAAGGATATTGGGCGG	TTAGAAGATGTCATGCCGCC	50.0	50.0	57.6	58.0	1117 bp	1.0	76.9