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ndhF-rpl32

SSC · 110869-111927 · 1059 bp

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Pi 0.000944

MarkerSeek score 59.30

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1059
variable_sites: 1
pi: 0.000944
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GTTGCCGTCGGAAAAAGAAG	CCGCCCAATATCCTTTCCTT	50.0	50.0	58.0	57.6	1239 bp	1.0	76.9
ndhF-rpl32_p2	TGCCGTCGGAAAAAGAAGAA	CCGCCCAATATCCTTTCCTT	45.0	50.0	58.0	57.6	1237 bp	1.0	76.8
ndhF-rpl32_p3	TTGCCGTCGGAAAAAGAAGA	CCGCCCAATATCCTTTCCTT	45.0	50.0	58.0	57.6	1238 bp	1.0	76.8
ndhF-rpl32_p4	GTTGCCGTCGGAAAAAGAAG	TGACAAAGCTTTTAACGCCG	50.0	45.0	58.0	57.6	1256 bp	1.0	76.7
ndhF-rpl32_p5	TGCCGTCGGAAAAAGAAGAA	TGACAAAGCTTTTAACGCCG	45.0	45.0	58.0	57.6	1254 bp	1.0	76.6