Hotspot

rps12-clpP

LSC · 69261-69421 · 161 bp

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Pi 0.0124

MarkerSeek score 59.60

Primer available yes

Label rps12-clpP

Feature Metadata

label_name: rps12-clpP
feature_type: igs
region: LSC
length_bp: 161
variable_sites: 2
pi: 0.0124
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-clpP_p1	GTACATGTTCCTCGACGCTG	TTATGTCAGCAACAGAGGCC	55.0	50.0	58.7	57.9	324 bp	1.0	73.7
rps12-clpP_p2	GTACATGTTCCTCGACGCTG	AACAGAGGCCCAAGCTTATG	55.0	50.0	58.7	57.9	314 bp	1.0	73.6
rps12-clpP_p3	GTACATGTTCCTCGACGCTG	CAACAGAGGCCCAAGCTTAT	55.0	50.0	58.7	57.9	315 bp	1.0	73.6
rps12-clpP_p4	AGTACATGTTCCTCGACGCT	TTATGTCAGCAACAGAGGCC	50.0	50.0	58.8	57.9	325 bp	1.0	73.0
rps12-clpP_p5	TACATGTTCCTCGACGCTGA	TTATGTCAGCAACAGAGGCC	50.0	50.0	58.8	57.9	323 bp	1.0	72.9