Hotspot

rps16

LSC · 4874-5964 · 1091 bp

Back to Nothosmyrnium

Pi 0.00826

MarkerSeek score 50.30

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1091
variable_sites: 9
pi: 0.00826
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	TTTTGTTTGATTAGGACCAAG	TGGGTTCCAAATTAGAGGAA	33.3	40.0	52.2	53.8	1141-1142 bp	1.0	40.7
rps16_p2	TTTTGTTTGATTAGGACCAAG	ATGGGTTCCAAATTAGAGGA	33.3	40.0	52.2	53.5	1142-1143 bp	1.0	40.7
rps16_p3	ATTTTGTTTGATTAGGACCAAG	TGGGTTCCAAATTAGAGGAA	31.8	40.0	52.9	53.8	1142-1143 bp	1.0	40.7
rps16_p4	TTTTGTTTGATTAGGACCAAG	ATGGGTTCCAAATTAGAGGAA	33.3	38.1	52.2	54.5	1142-1143 bp	1.0	40.7
rps16_p5	TTTTGTTTGATTAGGACCAAG	TGGGTTCCAAATTAGAGGAAT	33.3	38.1	52.2	54.5	1141-1142 bp	1.0	40.7