Hotspot

ndhF

SSC · 111839-114085 · 2247 bp

Back to Nothosmyrnium

Pi 0.00668

MarkerSeek score 61.00

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2247
variable_sites: 15
pi: 0.00668
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ATTGGAATTCCTGTCTATAGATT	GGGGGTAGGATCCGGTAAAT	30.4	55.0	52.7	58.3	2422 bp	0.5	20.0
ndhF_p2	ATTGGAATTCCTGTCTATAGATT	GGGTAGGATCCGGTAAATCG	30.4	55.0	52.7	57.3	2420 bp	0.5	20.0
ndhF_p3	ATTGGAATTCCTGTCTATAGATT	TTGAAAGGGGGTAGGATCCG	30.4	55.0	52.7	58.8	2428 bp	0.5	20.0
ndhF_p4	TTGGAATTCCTGTCTATAGATTA	GGGGGTAGGATCCGGTAAAT	30.4	55.0	52.1	58.3	2421 bp	0.5	20.0
ndhF_p5	ATTGGAATTCCTGTCTATAGATT	TGAAAGGGGGTAGGATCCG	30.4	57.9	52.7	58.1	2427 bp	0.5	20.0