Hotspot

ndhF-rpl32

SSC · 114086-115077 · 992 bp

Back to Nothosmyrnium

Pi 0.00715

MarkerSeek score 62.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 992
variable_sites: 7
pi: 0.00715
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GTTGCCGACGGAAAAAGAAG	TAAGGCTGCCCAATATCCCT	50.0	50.0	58.0	58.2	1177-1180 bp	1.0	79.8
ndhF-rpl32_p2	TGCCGACGGAAAAAGAAGAA	TAAGGCTGCCCAATATCCCT	45.0	50.0	58.0	58.2	1175-1178 bp	1.0	79.7
ndhF-rpl32_p3	TTGCCGACGGAAAAAGAAGA	TAAGGCTGCCCAATATCCCT	45.0	50.0	58.0	58.2	1176-1179 bp	1.0	79.7
ndhF-rpl32_p4	GTTGCCGACGGAAAAAGAAG	GACAACGCTTTTAAGGCTGC	50.0	50.0	58.0	58.3	1188-1191 bp	1.0	78.6
ndhF-rpl32_p5	AAAAGAAGAAGTCCCGCTCC	TAAGGCTGCCCAATATCCCT	50.0	50.0	57.8	58.2	1165-1168 bp	1.0	78.5