Hotspot

ycf15-trnV-GAC

LSC · 99427-100114 · 688 bp

Back to Nothosmyrnium

Pi 0.016
MarkerSeek score 53.40
Primer available yes
Label ycf15-trnV-GAC

Feature Metadata

label_name
ycf15-trnV-GAC
feature_type
igs
region
LSC
length_bp
688
variable_sites
11
pi
0.016
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf15-trnV-GAC_p1 GTACCGTGAGAGAAGCAAGG CTGCTGCTTGGTAGTAGTGG 55.0 55.0 58.0 58.0 1337-1347 bp 1.0 81.0
ycf15-trnV-GAC_p2 TGAGAGAAGCAAGGAGGTCA CTGCTGCTTGGTAGTAGTGG 50.0 55.0 58.0 58.0 1331-1341 bp 1.0 80.9
ycf15-trnV-GAC_p3 GTACCGTGAGAGAAGCAAGG AGGGAGAACCAGGTCTTACC 55.0 55.0 58.0 58.0 1195-1205 bp 1.0 80.9
ycf15-trnV-GAC_p4 GTACCGTGAGAGAAGCAAGG GGAGAACCAGGTCTTACCCT 55.0 55.0 58.0 58.0 1193-1203 bp 1.0 80.9
ycf15-trnV-GAC_p5 CATTGAGCGAAGGGTACGAA CTGCTGCTTGGTAGTAGTGG 50.0 55.0 58.0 58.0 1906 bp 0.5 59.8