Hotspot

rps12-ycf15

LSC · 98267-99234 · 968 bp

Back to Nothosmyrnium

Pi 0.011

MarkerSeek score 62.80

Primer available yes

Label rps12-ycf15

Feature Metadata

label_name: rps12-ycf15
feature_type: igs
region: LSC
length_bp: 968
variable_sites: 8
pi: 0.011
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.75
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-ycf15_p1	GCAACTTTACGTAGGGCAGA	TCTCCTCGGTCCACAGATAC	50.0	55.0	57.9	57.9	853-1097 bp	1.0	83.5
rps12-ycf15_p2	GCAACTTTACGTAGGGCAGA	TTTCTCCTCGGTCCACAGAT	50.0	50.0	57.9	58.1	855-1099 bp	1.0	83.5
rps12-ycf15_p3	CGTAGGGCAGAGTTTGGTTT	TCTCCTCGGTCCACAGATAC	50.0	55.0	58.1	57.9	844-1088 bp	1.0	83.4
rps12-ycf15_p4	CGTAGGGCAGAGTTTGGTTT	TTTCTCCTCGGTCCACAGAT	50.0	50.0	58.1	58.1	846-1090 bp	1.0	83.3
rps12-ycf15_p5	GCAACTTTACGTAGGGCAGA	AAATGTAAGACTGGCGCCAA	50.0	45.0	57.9	58.1	832-1076 bp	1.0	83.2