Hotspot

accD-psaI

LSC · 59760-60207 · 448 bp

Back to Nothosmyrnium

Pi 0.00915

MarkerSeek score 63.20

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 448
variable_sites: 4
pi: 0.00915
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GCTCCACGCTTTCTTTCCTT	AAAGAAGCCATTGCCATTGC	50.0	45.0	58.8	57.9	560-571 bp	1.0	75.2
accD-psaI_p2	GCTCCACGCTTTCTTTCCTT	TTGCCATTGCCGGAAATACT	50.0	45.0	58.8	58.2	550-561 bp	1.0	74.8
accD-psaI_p3	GCTCCACGCTTTCTTTCCT	AAAGAAGCCATTGCCATTGC	52.6	45.0	58.1	57.9	560-571 bp	1.0	72.8
accD-psaI_p4	TCCACGCTTTCTTTCCTTTGA	AAAGAAGCCATTGCCATTGC	42.9	45.0	58.1	57.9	558-569 bp	1.0	72.7
accD-psaI_p5	GAGTTATTTCAGCTCCACGC	AAAGAAGCCATTGCCATTGC	50.0	45.0	56.9	57.9	571-582 bp	1.0	72.5