Hotspot

atpA-atpF

LSC · 11641-11689 · 49 bp

Back to Nothosmyrnium

Pi 0.0408

MarkerSeek score 76.00

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 49
variable_sites: 2
pi: 0.0408
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	CCGTCACCTACTTGCAGTAC	AAGGGGCTCTAGGAACTCTG	55.0	55.0	58.0	58.1	260 bp	1.0	79.5
atpA-atpF_p2	CCGTCACCTACTTGCAGTAC	CAAGGGGCTCTAGGAACTCT	55.0	55.0	58.0	58.1	261 bp	1.0	79.5
atpA-atpF_p3	GTCACCTACTTGCAGTACGG	AAGGGGCTCTAGGAACTCTG	55.0	55.0	58.0	58.1	258 bp	1.0	79.5
atpA-atpF_p4	GTCACCTACTTGCAGTACGG	CAAGGGGCTCTAGGAACTCT	55.0	55.0	58.0	58.1	259 bp	1.0	79.5
atpA-atpF_p5	GTCACCTACTTGCAGTACGG	CCTTACAAGGGGCTCTAGGA	55.0	55.0	58.0	57.8	264 bp	1.0	79.1