Hotspot

trnK-UUU-rps16

LSC · 4142-4873 · 732 bp

Back to Nothosmyrnium

Pi 0.0169

MarkerSeek score 62.00

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 732
variable_sites: 12
pi: 0.0169
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	AGGAACTGTTCGGGATCTTT	50.0	45.0	58.2	56.5	789-812 bp	1.0	69.9
trnK-UUU-rps16_p2	AAGCCGAGTACTCTACCGTT	AGGAACTGTTCGGGATCTTT	50.0	45.0	58.2	56.5	788-811 bp	1.0	69.9
trnK-UUU-rps16_p3	TCCTAGCCGCACTTAAAAGC	AGGAACTGTTCGGGATCTTT	50.0	45.0	58.3	56.5	804-827 bp	1.0	69.0
trnK-UUU-rps16_p4	CCGCACTTAAAAGCCGAGTA	AGGAACTGTTCGGGATCTTT	50.0	45.0	58.3	56.5	798-821 bp	1.0	68.9
trnK-UUU-rps16_p5	AAGCCGAGTACTCTACCGTT	AGGAACTGTTCGGGATCTTTT	50.0	42.9	58.2	57.2	788-811 bp	1.0	67.9