Hotspot

rpoA

LSC · 79484-80503 · 1020 bp

Back to Nothoscordum

Pi 0.00196
MarkerSeek score 59.90
Primer available yes
Label rpoA

Feature Metadata

label_name
rpoA
feature_type
gene
region
LSC
length_bp
1020
variable_sites
2
pi
0.00196
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoA_p1 GAAAATACGGATTGCGCGTC CATAATGGATGTAGACCTCCT 50.0 42.9 58.3 53.9 1203-1223 bp 1.0 40.1
rpoA_p2 TTCGCGAAAATACGGATTGC CATAATGGATGTAGACCTCCT 45.0 42.9 57.6 53.9 1208-1228 bp 1.0 40.1
rpoA_p3 GAAAATACGGATTGCGCGTC TGGATGTAGACCTCCTAAAA 50.0 40.0 58.3 52.6 1198-1218 bp 1.0 40.1
rpoA_p4 GAAAATACGGATTGCGCGTC TGGATGTAGACCTCCTAAAAA 50.0 38.1 58.3 53.6 1198-1218 bp 1.0 40.1
rpoA_p5 TTCGCGAAAATACGGATTGC TGGATGTAGACCTCCTAAAA 45.0 40.0 57.6 52.6 1203-1223 bp 1.0 40.1