Hotspot

rpl16

LSC · 82796-84259 · 1464 bp

Back to Nothoscordum

Pi 0.00615

MarkerSeek score 62.40

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1464
variable_sites: 9
pi: 0.00615
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TCAATGCAAAGAATGAGGAA	TCCGTTAAATCAAACAAAAAGA	35.0	27.3	53.1	52.8	1664-1707 bp	1.0	40.5
rpl16_p2	TTCAATGCAAAGAATGAGGA	TCCGTTAAATCAAACAAAAAGA	35.0	27.3	53.1	52.8	1665-1708 bp	1.0	40.5
rpl16_p3	TCAATGCAAAGAATGAGGAAA	TCCGTTAAATCAAACAAAAAGA	33.3	27.3	54.0	52.8	1664-1707 bp	1.0	40.5
rpl16_p4	TTCAATGCAAAGAATGAGGAA	TCCGTTAAATCAAACAAAAAGA	33.3	27.3	54.0	52.8	1665-1708 bp	1.0	40.5
rpl16_p5	TTTCAATGCAAAGAATGAGGA	TCCGTTAAATCAAACAAAAAGA	33.3	27.3	54.0	52.8	1666-1709 bp	1.0	40.5