Hotspot

atpF

LSC · 11949-13401 · 1453 bp

Back to Nothoscordum

Pi 0.000697

MarkerSeek score 60.40

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1453
variable_sites: 1
pi: 0.000697
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	TCTTTTTCGGAAACAAAGGGA	AAAAAGGGCGAGTCAAGTGA	38.1	45.0	55.9	57.7	1569-1586 bp	1.0	53.6
atpF_p2	TCTTTTTCGGAAACAAAGGGA	GGGCGAGTCAAGTGATACAA	38.1	50.0	55.9	57.6	1564-1581 bp	1.0	53.3
atpF_p3	ACTAATTTCGTCAGCTCGAA	AAAAAGGGCGAGTCAAGTGA	40.0	45.0	54.9	57.7	1613-1630 bp	1.0	53.1
atpF_p4	ACTAATTTCGTCAGCTCGAA	GGGCGAGTCAAGTGATACAA	40.0	50.0	54.9	57.6	1608-1625 bp	1.0	52.8
atpF_p5	TCTTTTTCGGAAACAAAGGGA	AGGGCGAGTCAAGTGATACA	38.1	50.0	55.9	58.4	1565-1582 bp	1.0	52.8