Hotspot

rpl20-rps12

LSC · 70477-71220 · 744 bp

Back to Nothoscordum

Pi 0.00538

MarkerSeek score 55.30

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 744
variable_sites: 4
pi: 0.00538
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	GCCCCTATAAAGGTTGAGGC	AATATCACGAAATCCCCCGC	55.0	50.0	58.0	58.1	890 bp	1.0	79.4
rpl20-rps12_p2	GCCCCTATAAAGGTTGAGGC	TCGGGGAACATGTACTAGGG	55.0	55.0	58.0	58.2	849 bp	1.0	78.5
rpl20-rps12_p3	TTTTTGTTCGACGTCTCCGA	AATATCACGAAATCCCCCGC	45.0	50.0	57.8	58.1	859 bp	1.0	77.9
rpl20-rps12_p4	TTCGACGTCTCCGAGCTATA	AATATCACGAAATCCCCCGC	50.0	50.0	57.8	58.1	853 bp	1.0	77.7
rpl20-rps12_p5	TTTTGTTCGACGTCTCCGAG	AATATCACGAAATCCCCCGC	50.0	50.0	58.2	58.1	858 bp	1.0	77.6