Hotspot

rpl33-rps18

LSC · 69354-69524 · 171 bp

Back to Nothoscordum

Pi 0.024

MarkerSeek score 68.50

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 171
variable_sites: 4
pi: 0.024
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	TGTCGCTATTGTCACAAGCA	CAATTCGATCCCCCGATTCA	45.0	50.0	57.8	57.7	300-304 bp	1.0	78.8
rpl33-rps18_p2	TGTCGCTATTGTCACAAGCA	TCGATCCCCCGATTCAATTG	45.0	50.0	57.8	57.7	296-300 bp	1.0	78.8
rpl33-rps18_p3	TGTCGCTATTGTCACAAGCA	TCAATTCGATCCCCCGATTC	45.0	50.0	57.8	57.5	301-305 bp	1.0	76.5
rpl33-rps18_p4	TGTCGCTATTGTCACAAGCA	GGGAAAACGCCTACGAAAAG	45.0	50.0	57.8	57.4	275-279 bp	1.0	76.2
rpl33-rps18_p5	CGACACAATACACCCAGTCG	TCGATCCCCCGATTCAATTG	55.0	50.0	58.7	57.7	335-339 bp	1.0	74.6