Hotspot

ndhC-trnV_UAC

LSC · 51312-53141 · 1830 bp

Back to Nothoscordum

Pi 0.00328

MarkerSeek score 49.70

Primer available yes

Label ndhC-trnV_UAC

Feature Metadata

label_name: ndhC-trnV_UAC
feature_type: igs
region: LSC
length_bp: 1830
variable_sites: 6
pi: 0.00328
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV_UAC_p1	GGTCCTTCACTAATTGGGGC	GGTTCGAATCCGTATAGCCC	55.0	55.0	58.2	57.9	1965-1980 bp	1.0	77.8
ndhC-trnV_UAC_p2	GGTCCTTCACTAATTGGGGC	CGGTTCGAATCCGTATAGCC	55.0	55.0	58.2	58.3	1966-1981 bp	1.0	76.0
ndhC-trnV_UAC_p3	GTCCTTCACTAATTGGGGCT	GGTTCGAATCCGTATAGCCC	50.0	55.0	57.2	57.9	1964-1979 bp	1.0	73.2
ndhC-trnV_UAC_p4	CTGGTCCTTCACTAATTGGGG	GGTTCGAATCCGTATAGCCC	52.4	55.0	58.0	57.9	1967-1982 bp	1.0	71.6
ndhC-trnV_UAC_p5	GTCCTTCACTAATTGGGGCT	CGGTTCGAATCCGTATAGCC	50.0	55.0	57.2	58.3	1965-1980 bp	1.0	71.4