Hotspot

rps14-psaB

LSC · 37969-38101 · 133 bp

Back to Nothoscordum

Pi 0.015

MarkerSeek score 61.10

Primer available yes

Label rps14-psaB

Feature Metadata

label_name: rps14-psaB
feature_type: igs
region: LSC
length_bp: 133
variable_sites: 2
pi: 0.015
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps14-psaB_p1	TGTCGCTTCTTCTCCCTTTG	CCAGTGGCTCTTTCCATTGT	50.0	50.0	57.8	58.1	291 bp	1.0	78.5
rps14-psaB_p2	TGTCGCTTCTTCTCCCTTTG	GCAGCTTTCTTGATTGCCTC	50.0	50.0	57.8	57.7	216 bp	1.0	77.2
rps14-psaB_p3	TCTGTCGCTTCTTCTCCCTT	CCAGTGGCTCTTTCCATTGT	50.0	50.0	58.4	58.1	293 bp	1.0	76.9
rps14-psaB_p4	TTCTGTCGCTTCTTCTCCCT	CCAGTGGCTCTTTCCATTGT	50.0	50.0	58.4	58.1	294 bp	1.0	76.9
rps14-psaB_p5	TGTCGCTTCTTCTCCCTTTG	GCTCTTTCCATTGTGCAAGC	50.0	50.0	57.8	58.3	285 bp	1.0	76.9