Hotspot

trnS_UGA-psbZ

LSC · 36316-36647 · 332 bp

Back to Nothoscordum

Pi 0.00301
MarkerSeek score 54.90
Primer available yes
Label trnS_UGA-psbZ

Feature Metadata

label_name
trnS_UGA-psbZ
feature_type
igs
region
LSC
length_bp
332
variable_sites
1
pi
0.00301
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS_UGA-psbZ_p1 CAACCACTCAGCCATCTCTC ACCAACCATCAGAAGAAGCA 55.0 45.0 58.0 57.0 453-459 bp 1.0 72.3
trnS_UGA-psbZ_p2 GAGCTATCAACCACTCAGCC ACCAACCATCAGAAGAAGCA 55.0 45.0 58.1 57.0 460-466 bp 1.0 72.1
trnS_UGA-psbZ_p3 GGAGCTATCAACCACTCAGC ACCAACCATCAGAAGAAGCA 55.0 45.0 58.1 57.0 461-467 bp 1.0 72.1
trnS_UGA-psbZ_p4 CAACCACTCAGCCATCTCTC TGCAAAAACAGCCAATTGGA 55.0 40.0 58.0 57.0 386-392 bp 1.0 72.1
trnS_UGA-psbZ_p5 CAAGACCGGAGCTATCAACC ACCAACCATCAGAAGAAGCA 55.0 45.0 58.1 57.0 468-474 bp 1.0 72.0